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2009
Lam, H, Kostov Y, Rao G, Tolosa L.  2009.  A luminescence lifetime assisted ratiometric fluorimeter for biological applications.. The Review of scientific instruments. 80(12):124302. Abstract
2008
Badugu, R, Kostov Y, Rao G, Tolosa L.  2008.  Development and application of an excitation ratiometric optical pH sensor for bioprocess monitoring.. Biotechnology progress. 24(6):1393-401. Abstract
Lam, H, Kostov Y, Rao G, Tolosa L.  2008.  Low-cost optical lifetime assisted ratiometric glutamine sensor based on glutamine binding protein.. Analytical biochemistry. 383(1):61-7. Abstract
Ge, X, Rao G, Tolosa L.  2008.  On the Possibility of Real-Time Monitoring of Glucose in Cell Culture by Microdialysis Using a Fluorescent Glucose Binding Protein Sensor. Biotechnol. Prog.. 24:691-697. Abstract
2007
Ge, X, Lam HT, Modi S, LaCourse WR, Rao G, Tolosa L.  2007.  Comparing the Performance of the Optical Glucose Assay Based on the Glucose Binding Protein with High Performance Anion-exchange Chromatography with Pulsed Electrochemical Detection: Efforts to Design a Low-Cost Point-of-Care Glucose Sensor. J Diab Sci Tech. 1:864-72.
2006
Tolosa, L, Rao G, Geddes C, Lakowicz JR.  2006.  Binding Proteins as Glucose Sensors. Topics in Fluorescence Spectroscopy. 11
2005
Bartolome, A, Bardliving C, Rao G, Tolosa L.  2005.  Fatty acid sensor for low-cost lifetime-assisted ratiometric sensing using a fluorescent fatty acid binding protein. Anal Biochem. 345:133-9. Abstract
Kostov, YV, Smith DS, Tolosa L, Rao G, Gryczynski I, Gryczynski Z, Malicka J, Lakowicz JR.  2005.  Directional Surface Plasmon-Coupled Emission from a 3 nm Green Fluorescent Protein Monolayer. Biotechnol. Prog.. 21:1731-1735. Abstract
Tolosa, L, Kostov YV, Rao G, Thompson R.  2005.  Fluorescence Based Sensing for Bioprocess Monitoring.
2004
Ge, X, Tolosa L, Rao G.  2004.  Dual-labeled glucose binding protein for ratiometric measurements of glucose. Anal Chem. 76:1403-10. Abstract
2003
Tolosa, L, Ge X, Rao G.  2003.  Reagentless optical sensing of glutamine using a dual-emitting glutamine-binding protein. Anal Biochem. 314:199-205. Abstract
Ge, X, Tolosa L, Simpson J, Rao G.  2003.  Genetically engineered binding proteins as biosensors for fermentation and cell culture. Biotechnology and Bioengineering. 84:723-731. Abstract
2002
Tolosa, L, Kostov YV, Harms P, Rao G.  2002.  Noninvasive measurement of dissolved oxygen in shake flasks. Biotechnology and Bioengineering. 80:594-597. Abstract
1999
Tolosa, L, Gryczynski I, Eichhorn LR, Dattelbaum JD, Castellano FN, Rao G, Lakowicz JR.  1999.  Glucose sensor for low-cost lifetime-based sensing using a genetically engineered protein. Anal Biochem. 267:114-20. Abstract
Lakowicz, JR, Gryczynski I, Tolosa L, Dattelbaum JD, Castellano FN, Li L, Rao G.  1999.  Advances in Fluorescence Spectroscopy: Multi-Photon Excitation, Engineered Proteins, Modulation Sensing and Microsecond Rhenium Metal-Ligand Complexes. 95:179-96.
Lakowicz, JR, Gryczynski I, Gryczynski Z, Tolosa L, Randers-Eichhorn L, Rao G.  1999.  Polarization-Based Sensing of Glucose Using an Oriented Reference Film. J Biomed Optics. 4:443-9.
Lakowicz, JR, Gryczynski I, Gryczynski Z, Tolosa L, Dattelbaum JD, Rao G.  1999.  Polarization-Based Sensing with a Self-Referenced Sample. Appl Spec. 53:1149-57.
1998
Lakowicz, JR, Castellano FN, Dattelbaum JD, Tolosa L, Rao G, Gryczynski I.  1998.  Low-frequency modulation sensors using nanosecond fluorophores. Anal Chem. 70:5115-21. Abstract
1997
Tolosa, L, Szmacinski H, Rao G, Lakowicz JR.  1997.  Lifetime-based sensing of glucose using energy transfer with a long lifetime donor. Anal Biochem. 250:102-8. Abstract
Tolosa, L, Malak H, Rao G, Lakowicz JR.  1997.  Optical Assay for Glucose Based on the Luminescence Decay Time of the Long Wavelength Dye Cy5. Sensors and Actuators. B. Chemical. 45:93-9.
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